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王國禎 / Dr. Gou-Jen Wang

 

 

王國禎 / Dr. Gou-Jen Wang

工學院院長

Dean of College of Engineering

國立中興大學

National Chung-Hsing University

 Education:

  • PhD, Mechanical Engineering, UCLA

 Experiences:

  • Medal for the year 2017 - International Association of Advanced Materials (IAAM)

  • 13th Innovators: Four medals

  • Vice Director, Biotechnology Center, National Chung-Hsing University

  • Chairman, Graduate Institute of Biomedical Engineering, National Chung-Hsing University

 Abstract:

 

  Electrochemical analysis is a promising method for biosensing. In our current work, a highly sensitive nanostructured electro- chemical biosensor based on a 3D sensing element featuring uniformly deposited gold nanoparticles has been developed. The barrier-layer surface of an anodic aluminum oxide (AAO) membrane was adopted as the substrate. A gold thin-film was sputtered onto the substrate, followed by a uniform electrochemical deposition of gold nanoparticles (GNPs) on the gold thin-film. We have been collaborating with Taichung Veterans General Hospital, Academia Sinica, Kaohsiung Medical University, and orchid virus experts for successful detections of the dust mite antigen Der p2, allergy patient’s serum, allergy disease related gene mutations (SNP and Haplotype), dengue and Japanese encephalitis virus-receptor binding, hepatitis B virus DNA, Alzheimer's (AD) Aβ protein, and orchid virus using our nanostructured biosensors.

  In addition to the above nanostructured electrochemical biosensor, we have collabroated with Phonics International Semiconductor Inc. to develop a cost effective and high sensitive non-enzymatic glucose biosensor using a simple MEMS method. Measurements showed a 10.2-fold enhancement of the sensing area in comparison with a plain gold electrode. Actual detection of glucose demonstrated that the proposed non-enzymatic glucose biosensor can operate in a linear range of 55.6 M-13.89 mM. It had a sensitivity of 749.2 μA·mM−1·cm−2 and a detection limit of 9 M. When the glucose concentration is less than 100 mg/dL, the accuracy of the developed glucose biosensor reaches ±0.18 mg/dL, which is much better than the FDS and ISO 15197 standard of ±20 mg/dL and ±15 mg/dL, respectively.

 

 

 

 

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